Two splice variants of CDKL5 were cloned from a zebrafish cDNA library.
Three variants of amphiphysin 1 (Amph1), a possible target of CDKL5, were obtained.
Amph1 variants were significantly phosphorylated by CDKL5.
Colocalization of zebrafish CDKL5 and Amph1 was observed in living cells.
Variants of CDKL5 and Amph1 showed different spatial and temporal expressions.