Reversal of neurochemical alterations in the spinal dorsal horn and dorsal root ganglia by Mas-related gene (Mrg) receptors in a rat model of spinal nerve injury
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- 作者：Dongmei Wang ; Yaping Xue ; Yanhua Yan ; Minjie Lin ; Jiajia Yang ; Jianzhong Huang ; Yanguo Hong ; yhong@fjnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：BAM8&ndash ; 22 ; bovine adrenal medulla 8&ndash ; 22 ; CREB ; cyclic AMP response element-binding protein ; DMEM ; Dulbecco's modified Eagle's medium ; DRG ; dorsal root ganglion or ganglia ; ERK ; extracellular signal-regulated kinase ; GFAP ; glial fibrillary acidic protein ; GPCR ; G protein-coupled receptor ; IL-1β ; interleukin-1β ; i.t. ; intrathecal or intrathecally ; LPS ; lipopolysaccharide ; Mrg ; mas-related gene ; nNOS ; neuronal nitric oxide synthesis ; p ; phosphorylation ; PB ; phosphate buffer ; PBS ; phosphate
- 刊名：Neurobiology of Disease
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：91
- 期：Complete
- 页码：274-283
- 全文大小：2097 K
文摘
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Intrathecal (i.t.) administration of BAM8–22 inhibited mechanical allodynia in an opioid-independent manner.
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I.t. BAM8–22 suppressed expression of nNOS and p-CREB in the spinal dorsal horn.
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I.t. BAM8–22 reversed astrocytic activation and expression of interleukin-1β and p-ERK in the dorsal horn.
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I.t. BAM8–22 reversed the expression of fractalkine and IL-1β in dorsal root ganglion (DRG) neurons.
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The BAM8–22 exposure suppressed lipopolysaccharide-induced increase of nNOS and IL-1β in the DRG explant cultures.