Improving performance of a rapid electrochemical MRSA assay: Optimisation of assay conditions to achieve enhanced discrimination of clinically important DNA sequences under ambient conditions

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• 作者：D.K. Corrigan^a ; ^b ; ^c ; H. Schulze^b ; I Ciani^b ; G Henihan^b ; A.R. Mount^c ; T.T. Bachmann^b ; ^{till.bachmann@ed.ac.uk}
• 关键词：Electrochemistry ; DNA ; Gold electrodes ; Electrochemical impedance spectroscopy ; Biosensors ; MRSA ; Clinical detection ; Point of care ; Antimicrobial resistance ; Screen printed electrodes
• 刊名：Journal of Electroanalytical Chemistry
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：786
• 期：Complete
• 页码：58-62
• 全文大小：463 K
• 卷排序：786

文摘

A rapid electrochemical test for MRSA was optimised to improve electrode reproducibility and discrimination at ambient temperatures. Electrode reproducibility and sensor response was improved through the use of tris(2-carboxyethyl)phosphine (TCEP) in the probe immobilisation step. This was the case for both artificial targets and samples of genomic DNA extracted from MRSA. Room temperature discrimination was improved by the use of 40% formamide in the EIS measurement buffer. These findings will help to realise the application of electrochemical tests for point of care testing.