Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID

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• 作者：Xiaomei Bie ; Zhaoxin Lu ; Fengxia Lu
• 关键词：Identification ; Fengycin A ; Fengycin B ; Bacillus subtilis ; ESI-MS/CID
• 刊名：Journal of Microbiological Methods
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：79
• 期：3
• 页码：272-278
• 全文大小：1012 K

文摘

Bacillus subtilis fmbJ (CGMCC no.0943) was used to produce lipopeptides antibiotics fengycin. The fengycin mixture was precipitated with 6 N HCl and extracted by methanol. Electrospray ionization mass spectrometry/collision-induced dissociation (ESI-MS/CID) were used to analyze the fengycin mixture. In the ESI-MS/CID spectra of fengycin molecular ions, production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) were always detected. Therefore, these production ions (m/z 1080, m/z 966, m/z 1108 and m/z 994) could be used as fingerprints to quickly detect fengycin A and fengycin B. By the method of ESI-MS/CID, six homologues of fengycin A with C14 to 19 and four homologues of fengycin B with C14 to 17 were characterized. The method will be useful for rapidly determining whether a strain produces fengycin homologues.