Electrospun chitosan-graft-poly (-caprolactone)/poly (-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering

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• 作者：Honglin Chen ; Jin Huang ; Jiahui Yu ; Shiyuan Liu ; Ping Gu
• 关键词：Chitosan-graft-poly ( ; //www.sciencedirect.com/scidirimg/entities/25b.gif"" alt=""var epsilon"" title=""var epsilon"" border=""0"">-caprolactone)/poly ( ; //www.sciencedirect.com/scidirimg/entities/25b.gif"" alt=""var epsilon"" title
• 刊名：International Journal of Biological Macromolecules
• 出版年：2011
• 出版时间：1 January 2011
• 年：2011
• 卷：48
• 期：1
• 页码：13-19
• 全文大小：933 K

文摘

This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (-caprolactone) (CS-g-PCL) was synthesized with a facile one-step manner by grafting -caprolactone oligomers onto the hydroxyl groups of CS via ring-opening polymerization by using methanesulfonic acid as solvent and catalyst. And then, CS-g-PCL/PCL nanofibrous mats were obtained by electrospinning of CS-g-PCL/PCL mixed solution. Scanning electron microscopy (SEM) images showed that the morphologies and diameters of the nanofibers were mainly affected by the weight ratio of CS-g-PCL to PCL. The enrichment of amino groups on the nanofiber surface was confirmed by X-ray photoelectron spectroscopy (XPS). With the increase of CS-g-PCL in CS-g-PCL/PCL nanofiber, the content of amino groups on the nanofiber surface increased, which resulted in the increase of zeta-potential of nanofibers. Studies on cell–scaffold interaction were carried out by culturing mouse fibroblast cells (L929) on CS-g-PCL/PCL nanofibrous mats with various contents of CS-g-PCL by assessing the growth, proliferation and morphologies of cells. The results of MTS assay and SEM observation showed that CS-g-PCL/PCL (2/8) mats with a moderate surface zeta-potential (ζ = 3 mV) were the best in promoting the cell attachment and proliferation. Toluidine blue staining further confirmed that L929 cells grew well and exhibited a normal morphology on the CS-g-PCL/PCL (2/8) mats. These results suggested the potential utilization of CS-g-PCL/PCL (2/8) nanofibrous mats for skin tissue engineering.