Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity
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- 作者：Christopher W. Johnson ; Davinia Salvachú ; a ; Payal Khanna ; Holly Smith ; Darren J. Peterson ; Gregg T. Beckham ; gregg.beckham@nrel.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protocatechuate decarboxylase ; Pseudomonas Putida KT2440 ; cis ; cis-Muconate ; Muconic acid ; Lignin valorization
- 刊名：Metabolic Engineering Communications
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：3
- 期：Complete
- 页码：111-119
- 全文大小：1685 K
文摘
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We engineered Pseudomonas putida to produce muconate from p-coumarate and glucose.
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A protocatechuate decarboxylase was expressed with two associated proteins.
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Co-expression of these proteins reduced protocatechuate accumulation.
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Co-expression of these proteins reduced enhanced muconate production up to 3X.
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15.6 g/L muconate was produced from p-coumarate and 4.92 g/L from glucose