The influence of bovine casein-derived exorphins on mast cells in rodents
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- 作者：Stepnik ; M. ; Kurek ; M.
- 关键词：α ; -Casein ; 90&ndash ; 95 ; β ; -Casomorphin-7 ; Rat peritoneal mast cells ; Mouse bone marrow-derived &ldquo ; mucosal-like&rdquo ; mast cells ; β ; -Hexosaminidase activity ; Secretory effect of α ; -casein ; 90&ndash ; 95 ; α ; -Casé ; ine ; 90&ndash ; 9
- 刊名：Revue Francaise d'Allergologie et d'Immunologie Clinique
- 出版年：2002
- 出版时间：September, 2002
- 年：2002
- 卷：42
- 期：5
- 页码：447-453
- 全文大小：93.4 K
文摘
The secretagogue effects of α-casein (90–95) and β-casomorphin-7 on two populations of mast cells in rodents were investigated. Mast cells of “connective tissue type” were obtained from rat peritoneal cavity (PMC), and “mucosal-like type” mast cells (BMMC) were cultured from mouse bone marrow cells. β-Hexosaminidase activity was considered to be a marker of the cells’ secretory response. No reaction of BMMC on exorphins was found at up to millimolar concentrations. β-Casomorphin-7 and α-casein (90–95) exert secretagogue effect on PMC at milli- and micromolar concentrations, respectively. For this reason, further experiments were performed with α-casein (90–95) only. The PMC were activated in a non-cytotoxic, dose-, temperature-, and energy-dependent manner. Although PMC were responsive in a calcium and magnesium free buffer, the reaction was abolished when preincubation with EDTA was performed. Preincubation with opiate antagonist, naloxone, was effective only at millimolar concentration range. The significant decrease in PMC reactivity to α-casein (90–95) after preincubation with benzalkonium chloride suggests a peptidergic pathway of activation which involves a membrane-assisted but receptor-independent stimulation of Gi-like proteins.