An in vitro and in vivo investigation of the effects of diesel exhaust on human airway lining fluid antioxidants

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• 作者：Mudway ; Ian S. ; Stenfors ; Nikolai ; Duggan ; Sean T. ; Roxborough ; Heather ; Zielinski ; Hendrick ; et. al.
• 关键词：Diesel ; Nitrogen dioxide ; Antioxidants ; Iron ; Copper ; Semi volatile organic carbons ; Bronchoscopy ; Respiratory tract lining fluid
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2004
• 出版时间：March 1, 2004
• 年：2004
• 卷：423
• 期：1
• 页码：200-212
• 全文大小：343 K

文摘

Breathing high concentrations of diesel exhaust (DE) induces pulmonary inflammation, bronchoconstriction, increased airway reactivity, and oxidative stress in healthy subjects. To examine if these responses occur at environmentally relevant concentrations of DE, we exposed 25 healthy subjects to DE (PM₁₀ 100μg/m³, 0.6ppm NO₂ for 2-h) and filtered air on separate occasions. Immediately following DE exposure, subjects displayed an increase in subjective symptoms and a mild bronchoconstriction. Six hours following the cessation of DE exposure neither airway inflammation, nor antioxidant depletion (ascorbate, urate, and reduced glutathione), was seen at any level of the respiratory tract. Instead, an increased flux of reduced glutathione into the bronchial (p<0.01) and nasal airways (p<0.05) was observed. In separate, in vitro experiments, DE was found to have comparable oxidative activity to the transition metal rich residual oil fly ash (ROFA) particle, significantly depleting lung lining fluid ascorbic acid and reduced glutathione in a transition metal and superoxide-dependent mechanism. Together, these data indicate that even though DE has marked oxidative activity, this effect is not observed to any great extent in the airways of healthy subjects. We interpret these findings as being indicative that the antioxidant network at the air–lung interface in healthy subjects is capable of dealing with the oxidative challenge posed by DE at ambient concentrations.