In VitroSynthesis of 17,20β,21-Trihydroxy-4-pregnen-3-one by Ovaries of Turbot (Scophthalmus maximusL.) during Oocyte Maturation

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• 作者：Mugnier ; Chantal ; Gaignon ; Jean-Louis ; Fostier ; Alexis
• 刊名：General and Comparative Endocrinology
• 出版年：1997
• 出版时间：July, 1997
• 年：1997
• 卷：107
• 期：1
• 页码：63-73
• 全文大小：234 K

文摘

Ovaries from female turbot (Scophthalmus maximusL.), a serial spawner, were incubatedin vitrowith 17-hydroxy[1,2,6,7-³H]progesterone or [7-³H(N)]pregnenolone (P₅-³H) during the spawning season. Several metabolites comigrated on TLC and HPLC with known reference steroids and were identified after chemical reaction and crystallization. Incubation with P₅-³H generated many 4-ene steroids, accounting for 55 % of total radioactivity, indicating strong 3β-HSD activity. The major steroids produced by ovaries were testosterone, androstenedione, and 17,21-dihydroxy-4-pregnene-3,20-dione. In addition, 17,20α-dihydroxy-4-pregnen-3-one was also identified, while small quantities of 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) (maximum 1.7 % of the total radioactivity) were also synthesized. The identity of 20β-S was confirmed in incubates with nonlabeled 17-hydroxyprogesterone by mass spectrometry. Production of 17,20β-dihydroxy-4-pregnen-3-one was not apparent.