Synthesis of high 4-hydroxybutyrate copolymer by Cupriavidus sp. transformants using one-stage cultivation and mixed precursor substrates strategy

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• 作者：Ishak Muhammad Syafiq^a ; ^b ; Kai-Hee Huong^b ; K. Shantini^c ; S. Vigneswari^b ; ^d ; Nursolehah Abd Aziz^c ; Al-Ashraf Abdullah Amirul^b ; ^c ; ^e ; Kesaven Bhubalan^a ; ^b ; ^f ; ^{kesaven@umt.edu.my}
• 关键词：H1 ; 6  ; +  ; Lγ ; high concentration 1 ; 6-hexanediol and low concentration γ-butyrolactone ; L1 ; 6  ; +  ; Hγ ; low concentration 1 ; 6-hexanediol and high concentration γ-butyrolactone ; M1 ; 6  ; +  ; Mγ ; equal concentration 1 ; 6-hexanediol and γ-butyrolactone
• 刊名：Enzyme and Microbial Technology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：98
• 期：Complete
• 页码：1-8
• 全文大小：917 K
• 卷排序：98

文摘

High 4-hydroxybutyrate monomer accumulation by Cupriavidus sp. transformants is achieved. Mixed precursor substrate strategy exhibited pronounced synergistic effect. Two-fold increase in polyhydroxyalkanoate synthase activity in transformant strains. Ability to maintain high 4-hydroxybutyrate monomer composition in fermenter scale cultivation.