NS1 antigen: A new beam of light in the early diagnosis of dengue infection

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• 作者：Muhammad Suleman¹ ; ² ; Rani Faryal¹ ; Muhammad Masroor Alam² ; Salmaan Sharif² ; Shahzad Shaukat² ; Uzma Bashir Aamir² ; Adnan Khurshid² ; Mehar Angez² ; Massab Umair² ; Mian Muhammad Sufian² ; Yasir Arshad² ; Ghulam Mujtaba² ; Syed Sohail Zahoor Zaidi² ; ^{sohailz@live.com}
• 关键词：Dengue ; Pakistan ; Epidemiology ; ELISA ; NS1 antigen
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：12
• 页码：1212-1214
• 全文大小：351 K
• 卷排序：9

文摘

To evaluate NS1 antigen detection ELISA for the early laboratory diagnosis of dengue virus infection.MethodsThe present study was conducted to evaluate the overall positivity of NS1 antigen detection ELISA and its comparison with viral RNA detection via real time PCR and IgM antibodies detection by ELISA.ResultsA total of 1270 serum samples were tested 86% (1097/1270) were detected positive by one or more than one diagnostic test. Out of 1 270, 64% (807/1270) were positive by NS1 ELISA and 52% (662/1270), 51% (646/1270) were positive by real-time RT-PCR and IgM ELISA respectively.ConclusionsNS1 antigen detection ELISA is highly suitable diagnostic tools and it also has great value for use in outbreak and epidemic situation.