Profiling microRNAs and their targets in an important fleshy fruit: Tomato (Solanum lycopersicum)

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• 作者：Muhammad Din ; Muhammad Younas Khan Barozai
• 关键词：aly ; Arabidopsis lyrata ; ath ; Arabidopsis thaliana ; bdi ; Brachypodium distachyon ; BLAST ; Basic Local Alignment Search Tool ; dbEST ; Database of EST ; DCL1 ; Dicer-like Enzyme1 ; ESTs ; Expressed Sequence Tags ; gma ; Glycine max ; MFE ; Minimum Free Energy ; MIR ; MicroRNA Gene ; miRNA ; MicroRNA ; miRNA* ; Opposite Strand of miRNA ; mRNA ; Messenger RNA ; NCBI ; National Center for Biotechnology Information ; nt ; Nucleotide ; osa ; Oryza sativa ; Pol II ; RNA Polymerase II Enzyme ; Pre-miRNA ; Precursor-miRNA ; Pri-miRNA
• 刊名：Gene
• 出版年：10 February, 2014
• 年：2014
• 卷：535
• 期：2
• 页码：198-203
• 全文大小：414 K

文摘

Tomato (Solanum lycopersicum) is an important and the most useful plant based diet. It is widely used for its antioxidant property. Presently, only two digits, tomato microRNAs (miRNAs) are reported in miRBase: a miRNA database. This study is aimed to profile and characterize more miRNAs and their targets in tomato. A comprehensive comparative genomic approach is applied and a total of 109 new miRNAs belonging to 106 families are identified and characterized from the tomato expressed sequence tags (ESTs). All these potential miRNAs are profiled for the first time in tomato. The profiled miRNAs are also observed with stable stem-loop structures (Precursor-miRNAs), whose length ranges from 45to 329 nucleotides (nt) with an average of 125 nt. The mature miRNAs are found in the stem of pre-miRNAs and their length ranges from 19 to 24 nt with an average of 21 nt. Furthermore, twelve miRNAs are randomly selected and experimentally validated through RT-PCR. A total of 406 putative targets are also predicted for the newly 109 tomato miRNAs. These targets are involved in structural protein, metabolism, transcription factor, growth & development, stress related, signaling pathways, storage proteins and other vital processes. Some important proteins like; 9-cisepoxycarotenoid dioxygenase (NCED), transcription factor MYB, ATP-binding cassette transporters, terpen synthase, 14-3-3 and TIR-NBS proteins are also predicted as putative targets for tomato miRNAs. These findings improve a baseline data of miRNAs and their targets in tomato. This baseline data can be utilized to fine tune this important fleshy fruit for nutritional & antioxidant properties and also under biotic & abiotic stresses.