Transcriptome analysis of a CHO cell line expressing a recombinant therapeutic protein treated with inducers of protein expression
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- 作者：Dina Fomina-Yadlin ; Mirna Mujacic ; Kathy Maggiora ; Garrett Quesnell ; Ramsey Saleem ; Jeffrey T. McGrew ; jeff.mcgrew@justbiotherapeutics.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：CHO ; Chinese hamster ovary ; DHFR ; dihydrofolate reductase ; GO ; gene ontology ; GS ; glutamine synthetase ; GSEA ; gene set enrichment analysis ; HC ; heavy chain ; HMBA ; hexamethylene bisacetamide ; IgG1 ; human immunoglobulin G1 ; IRES ; internal ribosome entry site ; LC ; light chain ; mAb ; monoclonal antibody ; NaBu ; sodium butyrate ; PCA ; principal component analysis ; qP ; specific productivity ; RANK ; receptor activator of NF-Kappa B ; RNA- Seq ; next-generation RNA sequencing ; RPKM ; reads per kilobase of exon
- 刊名：Journal of Biotechnology
- 出版年：2015
- 出版时间：20 October 2015
- 年：2015
- 卷：212
- 期：Complete
- 页码：106-115
- 全文大小：1548 K
文摘
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RNA-Seq analysis was used to examine inducer effects on gene expression.
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Inducer treatment increased RANK–Fc mRNA from 16% up to 45% of total cellular mRNA.
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Specific productivity positively correlated to recombinant protein mRNA level.
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Selectable marker and RANK–Fc were 2 out of 7 mRNAs positively correlated to qP.
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Expression of many cell growth and housekeeping genes negatively correlated to qP.
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Transcript level of the recombinant protein is a major qP determinant.