- 作者：Mohd. Mujahid ; Hefazat H. Siddiqui ; Arshad Hussain ; Md. Sarfaraj Hussain
- 关键词：Anti-tubercular drugs ; Hepatoprotective ; Adenanthera pavonina ; Anti-oxidant ; Silymarin
- 刊名：Pharmacognosy Journal
- 出版年：November-December, 2013
- 年：2013
- 卷：5
- 期：6
- 页码：286-290
- 全文大小：545 K
Objective
The aim of the current study was to evaluate the hepatoprotective action of the leaves of Adenanthera pavonina against isoniazid (INH) and rifampicin (RIF)-induced liver damage in experimental animals.Methods
Five groups of six rats each were selected for the study. A methanolic (50%) extract of A.聽pavonina at a dose of 100 and 200聽mg/kg as well as silymarin 100聽mg/kg were administered orally once daily for 28 days in INH聽+聽RIF treated groups. The serum levels of glutamic oxaloacetic transaminase (SGOT), glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin, total protein, albumin and lactate聽dehydrogenase (LDH) were estimated along with activities of superoxide dismutase (SOD), catalase, glutathione, thiobarbituric acid reactive substances (TBARS). Histopathological analysis was carried out to assess injury to the liver tissue.
Result
The methanolic extract of A.聽pavonina was safe up to a dose of 2000聽mg/kg. The significantly elevated serum enzymatic activities of SGOT, SGPT, ALP, bilirubin and LDH due to INH聽+聽RIF treatment were restored to near normal in a dose dependent manner after the treatment with methanolic extract of leaves of A.聽pavonina. Also the increased level of total protein and albumin towards normal by extract of A.聽pavonina leaves. In the anti-oxidant studies a significant increase in the levels of glutathione, catalase and superoxide dismutase was observed. In addition, methanolic extract also significantly prevented the elevation of hepatic malondialdehyde formation in the liver of INH聽+聽RIF intoxicated rats in a dose dependent manner. The biochemical observations were supplemented with histopathological examination of rat liver sections.
Conclusions
These findings suggested that the methanolic extract of leaves of A.聽pavonina exhibited hepatoprotective effects against INH聽+聽RIF induced hepatic damage in rats as compared to standard drug silymarin.