Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes

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• 作者：Amit Kishore ; Monika Sodhi ; Keviletsu Khate ; Neha Kapila ; Parvesh Kumari ; Manishi Mukesh ; ^{mmukesh_26@hotmail.com} ; ^{mmukesh26@gmail.com}
• 关键词：Buffaloes ; Native cattle ; Heat stress ; Peripheral blood mononuclear cells (PBMCs) ; qRT-PCR ; Reference genes
• 刊名：Molecular and Cellular Probes
• 出版年：2013
• 出版时间：June-August, 2013
• 年：2013
• 卷：27
• 期：3-4
• 页码：140-144
• 全文大小：636 K

文摘

Eleven reference genes from different functional categories were assessed for their stable expression pattern in heat stressed PBMCs across Indian buffalo and cattle using GeNorm, NormFinder and BestKeeper algorithms. As the first report, we suggest B2M, RPS9 and RPS15a as suitable reference genes for accurate normalization of PBMC transcript data while the ACTB gene is not recommended.