Improved expression of ManB-1601 by using Hi-Control E. coli BL21 (DE3) cells.
Highest production (8406 U/ml) of recombinant endo-mannanase at flask level.
Solubilized-defatted flax seed meal based medium produced 5926 U/ml of ManB-1601.
Enhanced oil extraction from copra using ManB-1601.