Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models
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- 作者：Ví ; tor E. Santoa ; b ; 1 ; Marta F. Estradaa ; b ; 1 ; Sofia P. Rebeloa ; b ; Sofia Abreua ; b ; Inê ; s Silvaa ; b ; Catarina Pintoa ; b ; Susana C. Velosoa ; b ; Ana Teresa Serraa ; b ; Erwin Boghaertc ; Paula M. Alvesa ; b ; Catarina Britoa ; b ; anabrito@itqb.unl.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：In vitro cancer models ; Tumor spheroids ; Stirred-tank culture systems ; Drug discovery ; 3D cell culture
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 March 2016
- 年：2016
- 卷：221
- 期：Complete
- 页码：118-129
- 全文大小：2438 K
文摘
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Platform for generation of multicellular tumor spheroids in stirred-tank culture systems.
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High number of spheroids per run for feeding high-throughput screenings in pre-clinical research.
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Cells from distinct oncopathologies successfully aggregated and cultured by standardized protocols.
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Tumor spheroids with different morphologies (spherical and irregular) and size can be produced by manipulation of hydrodynamic conditions in the stirred-tank culture systems.
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Co-cultures with fibroblasts were also demonstrated with or without physical support from hydrogels.