Cell alignment induced by anisotropic electrospun fibrous scaffolds alone has limited effect on cardiomyocyte maturation
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- 作者：Jingjia Hana ; b ; Qingling Wua ; c ; Younan Xiab ; c ; d ; Mary B. Wagnera ; c ; Chunhui Xua ; b ; c ; chunhui.xu@emory.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：3-D ; three-dimensional ; bFGF ; human basic fibroblast growth factor ; BMP4 ; bone morphogenetic protein 4 ; CM ; cardiomyocyte ; ECM ; extracellular matrix ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; hPSC ; human pluripotent stem cell ; MEF ; mouse embryonic fibroblast ; MEA ; multielectrode array ; MHC ; myosin heavy chain ; MLC ; myosin light chain ; NGS ; normal goat serum ; PFA ; paraformaldehyde ; PCL ; polycaprolactone ; PU ; polyurethane ; RPMI ; Roswell Park Memorial Institute 1640 medium ; RT ; room temperat
- 刊名：Stem Cell Research
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：16
- 期：3
- 页码：740-750
- 全文大小：2232 K
文摘
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Aligned electrospun fibrous scaffolds induce efficient cell alignment.
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Cells on the aligned fibrous scaffolds have improved sarcomere organization.
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Cells on the aligned fibrous scaffolds have increased expression of cardiac genes.
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Cells on the aligned fibrous scaffolds have appropriate pharmacological responses.
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Cell alignment alone does not improve calcium handling properties.