Characterization of the Adhesive Mucilages Secreted by Live Diatom Cells using Atomic Force Microscopy

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• 作者：Michael J. Higgins ; Simon A. Crawford ; Paul Mulvaney ; Richard Wetherbee
• 关键词：atomic force microscopy ; bioadhesion ; biofouling EPS (extracelluar polymeric substances) ; diatoms ; secretion
• 刊名：Protist
• 出版年：2002
• 出版时间：March 2002
• 年：2002
• 卷：153
• 期：1
• 页码：25-38
• 全文大小：1893 K

文摘

Atomic Force Microscopy (AFM) resolved the topography and mechanical properties of two distinct adhesive mucilages secreted by the marine, fouling diatom Craspedostauros australis. Tapping mode images of live cells revealed a soft and cohesive outer mucilage layer that encased most of the diatom's siliceous wall, and force curves revealed an adhesive force of 3.58 nN. High loading force, contact mode imaging resulted in cantilever [cleaned] cell walls, which enabled the first direct observation of the active secretion of soft mucilage via pore openings. A second adhesive mucilage consisted of strands secreted at the raphe, a distinct slit in the silica wall involved in cell-substratum attachment and motility. Force measurements revealed a raphe adhesive strand(s) resistant to breaking forces up to 60 nN, and these strands could only be detached from the AFM cantilever probe using the manual stepper motor.