Cryogenic grinding with liquid nitrogen is a method of powdering plant biomass. Cryocrushing was used for the first time as pretreatment of lignocelluloses. Cryocrushing disorganized the fibers, without lignin destruction. Saccharification of eucalyptus sawdust and rice hull improved more than 10 times. Cryocrushing did not improve the saccharification of pure cellulose.