- 作者：Junichi Murakami ; MD ; Kazuhiro Ueda ; MD ; kaueda@c-able.ne.jp" class="auth_mail" title="E-mail the corresponding author ; Fumiho Sano ; MD ; Masataro Hayashi ; MD ; Arata Nishimoto ; PhD ; Kimikazu Hamano ; MD
- 关键词：Microenvironment ; Matrix metalloproteinases ; Extracellular matrix ; Lung cancer ; Pulmonary emphysema
- 刊名：Journal of Surgical Research
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：200
- 期：2
- 页码：690-697
- 全文大小：1772 K
Methods
The subjects included 48 patients with completely resected and pathologically confirmed stage I non–small cell lung cancer. Quantitative computed tomography was used to diagnose pulmonary emphysema, and immunohistochemical staining was performed to evaluate the matrix metalloproteinase (MMP) expression status in the intratumoral stromal cells as well as the microvessel density (MVD).
Results
Positive MMP-9 staining in the intratumoral stromal cells was confirmed in 17 (35%) of the 48 tumors. These 17 tumors were associated with a high MVD, frequent lymphovascular invasion, a high proliferative activity, and high postoperative recurrence rate (all, P < 0.05). The majority of the tumors (13 of 17) arose in patients with pulmonary emphysema (P = 0.02). Lung cancers arising from pulmonary emphysema were also associated with a high MVD, proliferative activity, and postoperative recurrence rate (all, P < 0.05).
Conclusions
The MMP-9 expression in intratumoral stromal cells is associated with the clinicopathologic aggressiveness of lung cancer and is predominantly identified in tumors arising in emphysematous lungs. Further studies regarding the biological links between the intratumoral and extratumoral microenvironment will help to explain why lung cancers originating in emphysematous lung tissues are associated with a poor prognosis.