Membranous expression of activated leukocyte cell adhesion molecule contributes to poor prognosis and malignant phenotypes of non-small-cell lung cancer

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• 作者：Futoshi Ishiguro ; MD^a ; ^b ; Hideki Murakami ; MD^a ; Tetsuya Mizuno ; MD^a ; ^b ; Makiko Fujii ; DDS^a ; Yutaka Kondo ; MD^a ; Noriyasu Usami ; MD^b ; Tetsuo Taniguchi ; MD^b ; Kohei Yokoi ; MD^b ; Hirotaka Osada ; MD^a ; ^c ; Yoshitaka Sekido ; MD^a ; ^c ; ^{ysekido@aichi-cc.jp}
• 关键词：Activated leukocyte cell adhesion molecule ; Immunohistochemistry ; Non&ndash ; small-cell lung cancer ; Prognosis
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：179
• 期：1
• 页码：24-32
• 全文大小：1295 K

文摘

Background

Activated leukocyte cell adhesion molecule (ALCAM) has been shown to correlate with the prognosis of patients with various types of human malignancies. However, the relationship between ALCAM expression and progression of non-small-cell lung cancer (NSCLC) has not been investigated. This study was designed to clarify the prognostic impact of ALCAM expression of NSCLC cells.

Materials and methods

The study population consisted of 147 NSCLC patients who underwent complete resection. We performed immunohistochemical staining for ALCAM expression and correlated this to the clinicopathologic parameters and patient survival. The ALCAM expression in NSCLC cell lines was analyzed using quantitative reverse transcription-polymerase chain reaction and Western blot analyses. ALCAM knockdown in NSCLC cell lines was performed with lentivirus-mediated short hairpin RNA transduction.

Results

Positive membranous and cytoplasmic ALCAM expressions were detected in 66?(44.9 % ) and 57 (38.8 % ) patients, respectively. A significant association of high membranous ALCAM expression with shortened overall survival (OS) was found (P = 0.009). However, patients with cytoplasmic staining of ALCAM showed no significantly shortened OS (P = 0.723). Multivariate analyses showed that membranous expression was adverse prognostic factors for OS (hazard ratio, 2.11; P = 0.046). ALCAM knockdown with short hairpin RNA suppressed cell migration and invasion of NSCLC cell lines in?vitro.

Conclusions

Strong membranous ALCAM expression is associated with a poor prognosis in patients with resected NSCLC, and overexpression of ALCAM causes malignant phenotypes of NSCLC.