Expression, purification, and characterization of human cystatin C monomers and oligomers
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- 作者：Tyler J. Perlenfein ; Regina M. Murphy ; regina.murphy@wisc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aβ ; beta-amyloid ; AD ; Alzheimer&rsquo ; s disease ; ANS ; 8-anilinonaphthalene-1-sulfonic acid ; catB ; cathepsin B ; CD ; circular dichroism ; cysC ; cystatin C ; DLS ; dynamic light scattering ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; MES ; 2-(N-morpholino)ethanesulfonic acid ; MWCO ; molecular weight cutoff ; PBSA ; phosphate buffered saline with azide ; SDS ; sodium dodecyl sulfate ; Z-FR-AMC ; N-carbobenzyloxy-phenylalanine-arginine-7-amino-4-methylcoumarin
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：35-43
- 全文大小：1227 K
文摘
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Successfully produced human cystatin C with a simple one-column purification scheme.
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Demonstrated oligomerization of cystatin C during concentration by ultrafiltration.
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Showed cystatin C oligomers but not monomers arrest beta-amyloid aggregation.