A human FSHB transgene encoding the double N-glycosylation mutant (Asn7Δ Asn24Δ) FSHβ subunit fails to rescue Fshb null mice
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- 作者：Huizhen Wanga ; Vladimir Butneve ; George R. Bousfielde ; T. Rajendra Kumara ; b ; c ; d ; tkumar@kumc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Gonadotropin ; Genetic rescue ; N-glycosylation ; Pituitary ; Ovary ; Testis
- 刊名：Molecular and Cellular Endocrinology
- 出版年：2016
- 出版时间：5 May 2016
- 年：2016
- 卷：426
- 期：Complete
- 页码：113-124
- 全文大小：4835 K
文摘
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In vivo functions of N-linked glycans on human FSHβ subunit tested in mouse models.
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HFSHBWT and HFSHBdgc (encodes a N-glycosylation mutant hFSHβ subunit) mice are generated on an Fshb−/− background.
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HFSHB transgenes are targeted to pituitary and specifically expressed in gonadotropes.
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HFSHBdgc encoded double N-glycosylation mutant human FSHβ subunit inefficiently assembles with mouse α-subunit.
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Double N-glycosylation mutant human FSHβ subunit containing FSH dimer is inefficiently secreted and does not rescue Fshb−/− mice.