Temporal and spatial requirements for Hoxa3 in mouse embryonic development

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• 作者：Jena L. Chojnowski¹ ; Heidi A. Trau² ; Kyoko Masuda³ ; Nancy R. Manley ; ^{nmanley@uga.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HOXA3 ; Temporal regulation ; Pharyngeal arches ; Organogenesis ; Thymus ; Thyroid
• 刊名：Developmental Biology
• 出版年：2016
• 出版时间：1 July 2016
• 年：2016
• 卷：415
• 期：1
• 页码：33-45
• 全文大小：8887 K

文摘

Hoxa3^null mice have severe defects in the development of pharyngeal organs including athymia, aparathyroidism, thyroid hypoplasia, and ultimobranchial body persistence, in addition to defects of the throat cartilages and cranial nerves. Some of the structures altered in the Hoxa3^null mutant embryos are anterior to the described Hoxa3 gene expression boundary: the thyroid, soft palate, and lesser hyoid horn. All of these structures develop over time and through the interactions of multiple cell types. To investigate the specific cellular targets for HOXA3 function in these structures across developmental time, we performed a comprehensive analysis of the temporal and tissue-specific requirements for Hoxa3, including a lineage analysis using Hoxa3^Cre. The combination of these approaches showed that HOXA3 functions in both a cell autonomous and non-cell autonomous manner during development of the 3rd and 4th arch derivatives, and functions in a neural crest cell (NCC)-specific, non-cell autonomous manner for structures that were Hoxa3-negative by lineage tracing. Our data indicate that HOXA3 is required for tissue organization and organ differentiation in endodermal cells (in the tracheal epithelium, thymus, and parathyroid), and contributes to organ migration and morphogenesis in NCCs. These data provide a detailed picture of where and when HOXA3 acts to promote the development of the diverse structures that are altered in the Hoxa3^null mutant. Data presented here, combined with our previous studies, indicate that the regionally restricted defects in Hoxa3 mutants do not reflect a role in positional identity (establishment of cell or tissue fate), but instead indicate a wider variety of functions including controlling distinct genetic programs for differentiation and morphogenesis in different cell types during development.