The objectives of this investigation were to identify the putative O-GlcNAc sites of 尾-catenin and the relevance of identified sites in the regulation of 尾-catenin's localization and transcriptional activity.
Missense mutations were introduced to potential O-GlcNAc sites of pEGFP-C2-N-Terminal- or pEGFP-C2-Wild Type-尾-catenin by site-directed mutagenesis. We determined the levels of O-GlcNAc-尾-catenin, subcellular localization, interaction with binding partners and transcriptional activity of the various constructs.
Serine 23 of 尾-catenin was determined as a site for O-GlcNAc modification which regulated its subcellular distribution, its interactions with cellular partners and consequently its transcriptional activity.
O-GlcNAcylation of Serine 23 is a novel regulatory modification for 尾-catenin's subcellular localization and transcriptional activity. This study is the first report to characterize site specific regulation of 尾-catenin by the O-GlcNAc modification.