- 作者：Jacqueline R. Ha ; Li Hao ; Geetha Venkateswaran ; Yu Hao Huang ; Elizabeth Garcia ; Sujata Persad
- 关键词：尾-Catenin ; O-GlcNAcylation ; Subcellular localization ; Transcriptional function
- 刊名：Experimental Cell Research
- 出版年：15 February, 2014
- 年：2014
- 卷：321
- 期：2
- 页码：153-166
- 全文大小：4432 K
Background
We have previously reported that 尾-catenin is post-translationally modified with a single O-linked attachment of 尾-N-acetyl-glucosamine (O-GlcNAc). We showed that O-GlcNAc regulated 尾-catenin's subcellular localization and transcriptional activity.Objective
The objectives of this investigation were to identify the putative O-GlcNAc sites of 尾-catenin and the relevance of identified sites in the regulation of 尾-catenin's localization and transcriptional activity.
Method
Missense mutations were introduced to potential O-GlcNAc sites of pEGFP-C2-N-Terminal- or pEGFP-C2-Wild Type-尾-catenin by site-directed mutagenesis. We determined the levels of O-GlcNAc-尾-catenin, subcellular localization, interaction with binding partners and transcriptional activity of the various constructs.
Results
Serine 23 of 尾-catenin was determined as a site for O-GlcNAc modification which regulated its subcellular distribution, its interactions with cellular partners and consequently its transcriptional activity.
Significance
O-GlcNAcylation of Serine 23 is a novel regulatory modification for 尾-catenin's subcellular localization and transcriptional activity. This study is the first report to characterize site specific regulation of 尾-catenin by the O-GlcNAc modification.