Involvement of ligninolytic enzymes of Myceliophthora vellerea HQ871747 in decolorization and complete mineralization of Reactive Blue 220

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• 作者：Vipul R. Patel ; Nikhil S. Bhatt ; H. B?Bhatt
• 关键词：Biodegradation ; Myceliophthora vellerea HQ871747 ; Immobilization ; HPLC ; NMR ; GC&ndash ; MS
• 刊名：Chemical Engineering Journal
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：233
• 期：Complete
• 页码：98-108
• 全文大小：2077 K

文摘

Myceliophthora vellerea HQ871747 was isolated from decayed wood sample and was subjected to phylogenetic analysis by using basidiomycete-specific ITS primers. Preferable physicochemical parameters were identified to achieve maximum enzyme and decolorization efficiency. M. vellerea HQ871747 tolerated 20 g L^?1 NaCl concentration without decrease in average rate of decolorization and showed more than 50 % decolorization up to 7th cycle of repetitive dye addition. It is also able to degrade mixture of various reactive, acidic, direct and disperse dyes. In immobilized packed-bed reactor 29-65 mg L^?1 h^?1 decolorization rate was achieved with >80 % COD removal, 154 and 75 UL^?1 MnP and laccase activity respectively. Purified MnP and laccase were monomeric protein with corresponding 37 and 25 kDa size on the SDS-PAGE. In this study it is proposed that the asymmetric cleavage of RB220 and HPLC, NMR and GC-MS confirmed formation of 2-diazo benzene sulfonic acid, naphthalene-2-sulfonic acid and 4-(4-diazenyl-5-oxo-4, 5-dihydro-1H-pyrazol-1-yl) benzene sulfonic acid. Treated wastewater was found to stimulate the growth of selected soil bacteria as well as plant growth.