An inducible expression system for high-level expression of recombinant proteins in slow growing mycobacteria
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- 作者：Lisa Leotta ; Joanne M. Spratt ; Carlyn U. Kong ; James A. Triccas ; jamie.triccas@sydney.edu.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mycobacterium ; hspX promoter ; Inducible expression ; Protein purification
- 刊名：Plasmid
- 出版年：2015
- 出版时间：September 2015
- 年：2015
- 卷：81
- 期：Complete
- 页码：27-31
- 全文大小：394 K
文摘
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The novel plasmid pJEX93 was constructed for inducible expression of recombinant proteins in slow growing mycobacteria.
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The Mycobacterium tuberculosis hspX promoter facilitates recombinant protein expression under low oxygen tension.
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Recombinant products can be purified from mycobacterial lysates by virtue of a C-terminal histidine tag.
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Recombinant proteins produced in this system display immunological and biochemical properties of their native counterparts.