Proteinase 3 mRNA expression is induced in monocytes but not in neutrophils of patients with cystic fibrosis
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- 作者：Just ; Jocelyne ; Moog-Lutz ; Christel ; Houzel-Charavel ; Anne ; Canteloup ; Sandrine ; Grimfeld ; Alain ; et. al.
- 关键词：Proteinase 3 ; Inflammation ; Lung disease ; Serine proteinase ; Myeloblastin ; Cystic fibrosis ; PR3 ; proteinase 3 ; CF ; cystic fibrosis ; NE ; neutrophil elastase ; SLPI ; secretory leucoprotease inhibitor ; MPO ; myeloperoxidase ; RT-PCR ; reverse transcription-polym
- 刊名：FEBS Letters
- 出版年：1999
- 出版时间：September 3, 1999
- 年：1999
- 卷：457
- 期：3
- 页码：437-440
- 全文大小：178 K
文摘
Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte-derived PR3 in CF-associated airway inflammation.