Nestin regulates neural stem cell migration via controlling the cell contractility

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• 作者：Sunxing Yan^a ; ¹ ; Panlong Li^a ; ^b ; ¹ ; Yong Wang^c ; ¹ ; Weihua Yu^a ; Aiping Qin^d ; Muyun Liu^a ; Andy Peng Xiang^a ; ^b ; ^e ; Wei Zhang^f ; ^{zhangw69@163.com" class="auth_mail" title="E-mail the corresponding author} ; Weiqiang Li^a ; ^e ; ^{liweiq6@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bleb ; blebbistatin ; CXCR4 ; chemokine receptor type 4 ; CXCR7 ; chemokine receptor type 7 ; EGFR ; epidermal growth factor receptor ; FAK ; focal adhension kinase ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; IF ; sintermediate filaments ; MLCK ; myosin light chain kinase ; MLCP ; myosin light chain phosphatase ; MRCK ; myotonic dystrophy kinase-related CDC42-binding kinase ; MRLC ; myosin regulatory light chain ; NM II ; non-muscle myosin II ; NSC ; neural stem cell ; PAK ; p21 activated kinase 1 ; PI3K ; phosphat
• 刊名：International Journal of Biochemistry and Cell Biology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：78
• 期：Complete
• 页码：349-360
• 全文大小：4390 K

文摘

Neural stem cells (NSCs) migration is essential for neurogenesis and neuroregeneration after brain injury. Nestin, a widely used marker of NSCs, is expressed abundantly in several cancers, where it may correlate with tumor migration and invasion. However, it is not yet known whether nestin participates in NSC migration. Here, we show that nestin down-regulation significantly inhibits the migration and contraction of murine neural stem cells, but does not obviously influence the proliferation, filamentous actin (F-actin) content, distribution or focal adhesion assembly of these cells. Mechanistically, nestin knockdown was found to affect the phosphorylation state of myosin regulatory light chain (MRLC) and regulate the activity of myosin light chain kinase (MLCK). Co-immunoprecipitation experiments showed that it interacts with MLCK and MRLC. Together, our results indicate that nestin may increase NSC motility via elevating MLCK activity through direct binding and provide new insight into the roles of nestin in NSC migration and repair.