Immobilized DM64 interacted with a wide variety of basic snake venom myotoxic PLA2. However, snake venom acidic PLA2 does not seem a preferential target for this inhibitor Soluble DM64 inhibited the cytotoxicity of several isolated basic myotoxins C-type lectins and CRISP may represent putative new targets for DM64 The results shed light on the structure-function relationship of DM64 This affinity-based approach can be useful in the search for novel toxin components