Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line

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• 作者：Nadine Platet ; Jean-Franç ; ois Mayol ; Franç ; ois Berger ; Francis Hé ; rodin ; Didier Wion
• 关键词：Cancer stem cell ; Side population ; Hoechst 33342 ; Glioma
• 刊名：FEBS Letters
• 出版年：2007
• 出版时间：3 April 2007
• 年：2007
• 卷：581
• 期：7
• 页码：1435-1440
• 全文大小：606 K

文摘

Using the C6 glioma cell as a paradigm, we found that (i) the clonogenicity of C6 cells is several orders of magnitude higher than the percentage of SP cells; (ii) non-SP cells are able to generate SP cells, and conversely SP cells generate non-SP cells; (iii) non-SP sorted cells behave as tumorigenic cells. Hence, in C6 cells cultured in serum-containing medium, SP cells can be generated from non-SP cells. This dynamic equilibrium explains in C6 cells the maintenance of the SP phenotype with cell passaging and demonstrates the existence of tumorigenic non-SP cells.