In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells

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• 作者：Yukiko Ishikura¹ ; ² ; Yukihiro Yabuta¹ ; ² ; Hiroshi Ohta¹ ; ² ; Katsuhiko Hayashi¹ ; ³ ; ⁴ ; Tomonori Nakamura¹ ; ² ; Ikuhiro Okamoto¹ ; ² ; Takuya Yamamoto⁵ ; ⁶ ; ⁷ ; Kazuki Kurimoto¹ ; ² ; Kenjiro Shirane⁸ ; ⁹ ; Hiroyuki Sasaki⁸ ; ⁹ ; Mitinori Saitou¹ ; ² ; ⁵ ; ⁶ ; ¹⁰ ; ^{saitou@anat2.med.kyoto-u.ac.jp}
• 关键词：spermatogonial stem cells ; embryonic stem cells ; primordial germ cells ; testes ; DNA methylation ; epigenetic reprogramming
• 刊名：Cell Reports
• 出版年：2016
• 出版时间：6 December 2016
• 年：2016
• 卷：17
• 期：10
• 页码：2789-2804
• 全文大小：11636 K
• 卷排序：17

文摘

In vitro derivation of mouse germline stem cell-like cells (GSCLCs) from ESCs GSCLCs colonize adult testes and contribute to spermatogenesis and fertile offspring GSCLCs show aberrant DNA methylation at vulnerable regulatory elements Spermatogonial stem cell induction relies on faithful regulation of DNA methylation