Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy

详细信息    查看全文

• 作者：Dong Nyoung Heo^a ; ¹ ; Dae Hyeok Yang^a ; ¹ ; Ho-Jin Moon^a ; Jung Bok Lee^a ; Min Soo Bae^a ; Sang Cheon Lee^a ; Won Jun Lee^b ; In-Cheol Sun^c ; Il Keun Kwon^a ; ^{kwoni@khu.ac.kr}
• 关键词：Gold nanoparticles ; Paclitaxel ; Biotin ; Beta-cyclodextrin ; Theranostic agents
• 刊名：Biomaterials
• 出版年：2012
• 出版时间：January, 2012
• 年：2012
• 卷：33
• 期：3
• 页码：856-866
• 全文大小：1K

文摘

We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin, paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (¦Â-CD) (AuNP-5?/strong>) can be useful as a theranostic agent for cancer therapy without the cytotoxic effect on normal cells. Prior to surface-functionalizing AuNPs, the cytotoxicity of the nanoparticles was evaluated, followed by their cytocompatibility. PTX, an anti-cancer agent, formed inclusion complexations with ¦Â-CD conjugated AuNPs, and effectively released from the AuNP-2?/strong> surface-functionalized with PEG, beta-cyclodextrin (¦Â-CD) and paclitaxel (PTX) using the intracellular glutathione (GSH) level (10?mm). Two types of AuNP-4 surface-functionalized with PEG and rhodamine B linked ¦Â-CD and AuNP-5 surface-functionalized PEG, biotin and rhodamine B linked ¦Â-CD were used for evaluating their specific interaction on cancer cells such as HeLa, A549 and MG63. These were also tested against normal NIH3T3 cell, determining that the AuNP-5 was more effectively involved with the cancer cells. Confocal laser scanning microscopy (CLSM), fluorescence-activated cell-sorting (FACS) and cell viability analyses showed that the AuNP-5?/strong> plays a significant role in the diagnosis and therapy of the cancer cells, and may be used in theranostic agents.