Fabrication of gelatin nanopatterns for cell culture studies

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• 作者：S.S. Li ; J. Shi ; L. Liu ; J.J. Li ; L.M. Jiang ; C.X. Luo ; K. Kamei ; Y. Chen
• 关键词：Gelatin ; Gel-embossing ; Cell culture ; Nanopatterns
• 刊名：Microelectronic Engineering
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：110
• 期：Complete
• 页码：70-74
• 全文大小：1292 K

文摘

We developed a gel-embossing technique which could be used to fabricate gelatin nanopatterns for cell culture studies. A gel of gelatin solution was dropped on the surface of a glass slide and embossed using a gas permeable stamp made of polydimethylsiloxane (PDMS). After drying in a desiccator, nanostructure patterns could be replicated from the stamp into the gelatin layer. After crosslinking in a 10 % formaldehyde solution in phosphate buffered saline (PBS), the embossed gelatin layer could be used for cell culture studies. The gel-embossed patterns before and after crosslinking were characterized by atomic force microscopy, revealing significant changes of the pattern morphology introduced by both gel-emboss and crosslink processes. Nonetheless, strong effects of cell elongation and fast cell growth were observed for the proof-of-concept using NIH-3T3 cells cultured on gelatin nanogrooves.