Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2¡ä-furil, a novel fluorogenic reagent
详细信息 查看全文
- 作者：Marwa Fathy Bakr Ali ; Naoya Kishikawa ; Kaname Ohyama ; Horria Abdel-Mageed Mohamed ; Hanaa Mohamed Abdel-Wadood ; Ashraf Mohamed Mohamed ; Naotaka Kuroda
- 关键词：Pre-column fluorogenic derivatization ; 1 ; 2-Di(2-furyl)-1 ; 2-ethanedione (2 ; 2&prime ; -furil) ; Difurylimidazole derivatives ; Aliphatic aldehydes (lipid peroxidation) ; Serum analysis
- 刊名：Journal of Chromatography A
- 出版年：2013
- 出版时间：26 July, 2013
- 年：2013
- 卷：1300
- 期：Complete
- 页码：199-203
- 全文大小：837 K
文摘
A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2¡ä-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v % ). The detection limits were from 0.19 to 0.50 nM (1-10 fmol/injection) at a signal-to-noise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.