Phosphorylation of retinoblastoma protein at apoptotic cell death in rat neuroblastoma B50 cells

详细信息    查看全文

• 作者：Honma ; Naoyuki ; Hosono ; Yoshie ; Kishimoto ; Takeo ; Hisanaga ; Shin-ichi
• 关键词：Apoptosis ; Cyclin-dependent kinases ; Neuroblastoma ; Retinoblastoma protein ; Phosphorylation
• 刊名：Neuroscience Letters
• 出版年：1997
• 出版时间：October 10, 1997
• 年：1997
• 卷：235
• 期：1-2
• 页码：45-48
• 全文大小：457 K

文摘

Phosphorylation of the retinoblastoma protein (RB) was observed during apoptosis of B50 neuroblastoma cells following induction by dibutyryl cAMP, after differentiation into neurons, or by cycloheximide during proliferation. A weak but distinct increase in a RB and histone H1 kinase activity was detected at the time of RB phosphorylation. However, the RB kinase appeared to correspond to neither p34^cdc2 kinase, CDK2 nor CDK5 because it was not inhibited by butyrolactone I, an inhibitor for them. Expression of CDK4 and 6 along with several cyclins also did not coincide with the appearance of phosphorylated RB in the apoptotic process.