N-Myc knockdown and apigenin treatment controlled growth of malignant neuroblastoma cells having N-Myc amplification

详细信息    查看全文

• 作者：Md. Motarab Hossain ; Naren L. Banik ; Swapan K. Ray
• 关键词：APG ; apigenin ; b-FGF ; basic fibroblast growth factor ; BSA ; bovine serum albumin ; CTL ; control ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; FITC ; fluorescein isothocyanate ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; HRP ; horseradish peroxidase ; hTERT
• 刊名：Gene
• 出版年：2013
• 出版时间：15 October, 2013
• 年：2013
• 卷：529
• 期：1
• 页码：27-36
• 全文大小：2471 K

文摘

Malignant neuroblastomas mostly occur in children and are frequently associated with N-Myc amplification. Oncogene amplification, which is selective increase in copy number of the oncogene, provides survival advantages in solid tumors including malignant neuroblastoma. We have decreased expression of N-Myc oncogene using short hairpin RNA (shRNA) plasmid to increase anti-tumor efficacy of the isoflavonoid apigenin (APG) in human malignant neuroblastoma SK-N-DZ and SK-N-BE2 cell lines that harbor N-Myc amplification. N-Myc knockdown induced morphological and biochemical features of neuronal differentiation. Combination of N-Myc knockdown and APG most effectively induced morphological and biochemical features of apoptotic death. This combination therapy also prevented cell migration and decreased N-Myc driven survival, angiogenic, and invasive factors. Collectively, N-Myc knockdown and APG treatment is a promising strategy for controlling the growth of human malignant neuroblastoma cell lines that harbor N-Myc amplification.