- 作者：Mushtaq D. Adil ; Peerzada Kaiser ; Naresh K. Satti ; Afzal M. Zargar ; Ram A. Vishwakarma ; Sheikh A. Tasduq
- 关键词：Skin fibroblast ; UVB ; Photo-aging ; Emblica officinalis
- 刊名：Journal of Ethnopharmacology
- 出版年：2010
- 出版时间：28 October 2010
- 年：2010
- 卷：132
- 期：1
- 页码：109-114
- 全文大小：530 K
Ethnopharmacological relevanceEmblica officinalis fruit (EO), commonly known as Amla is a reputed traditional medicine and functional food used in Indian subcontinent. It has long been used in Indian folk medicine to treat liver diseases, stomach ulcers, inflammatory diseases, metabolic disorders, geriatric complaints, skin disorders and beauty care.Aim of the study
Recently, it has been shown to promote pro-collagen content and inhibit matrix metalloproteinase levels in skin fibroblast. The aim of the present study was to investigate the efficacy of EO to inhibit UVB-induced photo-aging in human skin fibroblasts.
Materials and methods
Mitochondrial activity of human skin fibroblasts was measured by MTT-assay. Quantifications of pro-collagen 1 and matrix metalloproteinase 1 (MMP-1) release were performed by immunoassay techniques. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Cell cycle analysis was performed by flowcytometry using propidium iodide.
Results
EO stimulated, the otherwise UVB inhibited cellular proliferation and protected pro-collagen 1 against UVB-induced depletion via inhibition of UVB-induced MMP-1 in skin fibroblasts (10–40 μg/mL, p > 0.001). EO exhibited inhibitory activity of hyaluronidase (10–40 μg/mL, p > 0.001). Treatment with EO also prevented UVB disturbed cell cycle to normal phase.
Conclusion
The results of the present study suggests that EO effectively inhibits UVB-induced photo-aging in human skin fibroblast via its strong ROS scavenging ability and its therapeutic and cosmetic applications remain to be explored.