MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

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• 作者：Stefan M.K. Davies ; Maria I.G. Lopez Sanchez ; Reena Narsai ; Anne-Marie J. Shearwood ; Muhammad F.M. Razif ; Ian D. Small ; James Whelan ; Oliver Rackham ; Aleks ; ra Filipovska
• 关键词：PPR ; pentatricopeptide repeat ; MRPS27 ; mitochondrial ribosomal protein of the small subunit 27 ; TAP ; tandem affinity purification
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：19 October, 2012
• 年：2012
• 卷：586
• 期：20
• 页码：3555-3561
• 全文大小：850 K

文摘

Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA^Glu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.

Structured summary of protein interactions

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