Oleacein enhances anti-inflammatory activity of human macrophages by increasing CD163 receptor expression

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• 作者：Agnieszka Filipek^a ; Monika E. Czerwińska^a ; Anna K. Kiss^a ; Małgorzata Wrzosek^b ; Marek Naruszewicz^a ; ^{marek.naruszewicz@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author} ; ^{marek.naruszewicz@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Oleacein ; Macrophages ; CD163 ; Carotid plaque ; Atherosclerosis
• 刊名：Phytomedicine
• 出版年：2015
• 出版时间：15 December 2015
• 年：2015
• 卷：22
• 期：14
• 页码：1255-1261
• 全文大小：1281 K

文摘

Oleacein (dialdehydic form of decarboxymethyl elenolic acid linked to hydroxytyrosol; 3,4-DHPEA-EDA) have been proven to possess antioxidant and anti-inflammatory activity.

Purpose

In this study, we examined whether oleacein could increase CD163 and IL-10 receptor expression as well as HO-1 intracellular secretion in human macrophages.

Methods

Effect of oleacein (10 and 20 μmol/l) or oleacein together with complexes of haemoglobin (Hb) and haptoglobin 1-1 (Hp11) or haptoglobin 2-2 (Hp22) on expression of IL-10 and CD163 receptor was determined by Flow Cytometry. Expression of CD163mRNA was measured by real-time quantitative RT-PCR. Heme oxygenase 1 (HO-1) intracellular secretion in macrophages was investigated by enzyme-linked immunosorbent assay (ELISA).

Results

Oleacein (OC) together with complexes HbHp11 or HbHp22 stimulated the expression of CD163 (30-100-fold), IL-10 (170-300-fold) and HO-1 secretion (60-130-fold) after 5 days of coincubation. The 2-fold (24 h), 4-fold (48 h) increase of CD163 mRNA level and its final (72 h) decrease was also observed.

Conclusion

Our results suggested that oleacein enhances anti-inflammatory activity of complexes haemoglobin with haptoglobin 1-1 and 2-2 and could play a potential role in the prevention of inflammatory disease related to atherosclerosis.