Purification and primary structure of a novel mannose-specific lectin from Centrolobium microchaete Mart seeds

详细信息    查看全文

• 作者：Mayron Alves de Vasconcelos^a ; ^{mayronvasconcelos@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Ana Cecí ; lia Alves^b ; ² ; Rô ; mulo Farias Carneiro^c ; ² ; Artur Hermano Sampaio Dias^b ; ² ; Francisco William Viana Martins^b ; ³ ; Joã ; o Batista Cajazeiras^b ; ² ; Celso Shiniti Nagano^c ; ³ ; Edson Holanda Teixeira^a ; ¹ ; Kyria Santiago do Nascimento^b ; ² ; Benildo Sousa Cavada^b ; ² ; ^{bscavada@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Plant lectins ; Centrolobium microchaete ; Dalbergieae ; Primary structure
• 刊名：International Journal of Biological Macromolecules
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：81
• 期：Complete
• 页码：600-607
• 全文大小：1694 K

文摘

This study aimed to purify and characterize a novel mannose-binding lectin from the seeds of Centrolobium microchaete. Centrolobium microchaete lectin (CML) was purified by affinity chromatography in mannose-Sepharose-4B column. CML agglutinated rabbit erythrocytes and was inhibited by D-mannose, α-methyl-D-mannoside, D-glucose, N-Acetyl-D-glucosamine and sucrose. The lectin was stable at pH 7.0 and 8.0 and temperatures up to 60 °C. The monomeric form of CML showed approximately 28 kDa, and its native form is probably a homodimer, as determined by gel filtration chromatography. The primary structure of CML was determined by tandem mass spectrometry that showed CML as a protein with two distinct forms (isolectins CML-1 and CML-2) with 246 and 247 residues, respectively. CML-2 possesses one residue of Asn more than CML-1 in C-terminal. The primary structure of CML agrees with the molecular weights found by electrospray ionization mass spectrometry: 27,224 and 27,338 Da for CML-1 and CML-2, respectively. CML is a metal-dependent glycoprotein. Moreover, the glycan composition of CML and its structure were predicted.