Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis

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• 作者：Ito Liberato Barroso-Neto ; Rafael Concei莽茫o Sim玫es ; Bruno Anderson Matias Rocha ; Maria Julia Barbosa Bezerra ; Francisco Nascimento Pereira-Junior ; Vinicius Jos茅 Silva Osterne ; Kyria Santiago Nascimento ; Celso Shiniti Nagano ; Plinio Delatorre ; Maria Gon莽alves Pereira ; Alana Freitas Pires ; Alex ; re Hol ; a Sampaio ; Ana Maria Sampaio Assreuy ; Benildo Sousa Cavada
• 关键词：Canavalia grandiflora ; Lectin ; ConA-like ; Vasorelaxation ; Structure
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：1 February, 2014
• 年：2014
• 卷：543
• 期：Complete
• 页码：31-39
• 全文大小：3470 K

文摘

Lectins are comprised of a large family of proteins capable of the specific and reversible recognition of carbohydrates. Legume lectins, the most studied plant lectins, show high structural similarity, but with modifications that imply a variation in the intensity of some biological activities. In this work, the primary and tertiary structures of Canavalia grandiflora (ConGF) were determined. ConGF, a lectin isolated from C. grandiflora seeds, is able to induce relaxant activity in rat aortic rings. The complete sequence of ConGF comprises 237 amino acids. This particular protein has primary sequence variations commonly found in lectins from Dioclea and Canavalia genera. The protein structure was solved at 2.3 脜 resolution by X-ray crystallography. An X-Man molecule was modeled into the carbohydrate recognition domain. Still, ConGF (30 and 100 渭g mL^鈭?) elicited 25% of vasorelaxation (IC₅₀ = 34.48 卤 5.07 渭g mL^鈭?) in endothelialized aortic rings. A nonselective inhibitor of nitric oxide blocked ConGF relaxant effect, showing mediation by nitric oxide. Key distances between ConGF carbohydrate recognition domain residues were determined in order to explain this effect, in turn revealing some structural aspects that could differentiate lectins from the Canavalia genera with respect to different efficacy in vasorelaxant effect.