In vivo quantification of 5-HT_1A–[¹⁸F]MPPF interactions in rats using the YAP-(S)PET scanner and a β-microprobe

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• 作者：Philippe Millet ; Marcelle Moulin ; Antonietta Bartoli ; Alberto Del Guerra ; Nathalie Ginovart ; Laurent Lemoucheux ; Stephano Buono ; Daniel Fagret ; Yves Charnay ; Vicente Ibá ; ñ ; ez
• 关键词：β ; -Microprobe ; YAP-(S)PET ; [¹⁸F]MPPF ; 5-HT_1A ; Compartmental model
• 刊名：NeuroImage
• 出版年：2008
• 出版时间：1 July 2008
• 年：2008
• 卷：41
• 期：3
• 页码：823-834
• 全文大小：1267 K

文摘

We performed full modeling analysis of 5-HT_1A–[¹⁸F]MPPF interactions using the β-microprobe (βP) and a YAP-(S)PET scanner. Sixteen Wistar rats were used for βP (n = 5) and YAP-(S)PET (n = 5) acquisitions and metabolite studies (n = 6). Time–concentration curves were obtained in the hippocampus, raphe dorsalis, frontal cortex and cerebellum, using three injections of [¹⁸F]MPPF at different specific activities. B′_max values were estimated from a two (2T-5k)- and three (3T-7k)-tissue-compartment model with βP and YAP-(S)PET time–concentration curves. The simplified reference tissue model (SRTM) was used to estimate binding potential (BP_SRTM) values from data obtained with the first injection and the cerebellum as the reference region. Overall, the 3T-7k model provided a better fit than the 2T-5k model, as evaluated from AIC criteria in all experiments. The rank order of receptor density (B′_max) values was as follows: hippocampus > raphe ≈ frontal cortex > cerebellum. Non-negligible specific binding was observed in the cerebellum (B′_max (βP) = 1.5 ± 0.9 pmol/ml). Significant correlations (p < 0.001) between B′_max and BP_SRTM values were evident with both βP (r = 0.895) and YAP-(S)PET (r = 0.695). The YAP-(S)PET system underestimated the [¹⁸F]MPPF binding levels in brain due to limited resolution (i.e. partial volume), but led to similar conclusions.