To im
prove the mechanical
pro
perties and biocom
patibility of collagen I matrix, a novel and facile strategy was develo
ped to modify
porcine acellular
dermal matrix (PADM) via do
pamine self-
polymerization followed by collagen immobilization to enhance the biological, mechanical and
physicochemical
pro
perties of PADM. Mechanism study indicated that the
polymerization of do
pamine onto PADM surface could be regulated by controlling the amount of hydrogen bonds forming between
phenol hydroxyl (C
der="0" alt="single bond" data-inlimg="/entities/sbnd" src="/sd/grey_
pxl.gif" class="gly
phImg imgLazyJSB">
pt>der="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">pt>OH) and nitrogen atom (N
der="0" alt="single bond" data-inlimg="/entities/sbnd" src="/sd/grey_
pxl.gif" class="gly
phImg imgLazyJSB">
pt>der="0" alt="single bond" src="http://cdn.els-cdn.com/sd/entities/sbnd" class="glyphImg">pt>C
der="0" alt="double bond; length as m-dash" data-inlimg="/entities/dbnd" src="/sd/grey_
pxl.gif" class="gly
phImg imgLazyJSB">
pt>der="0" alt="double bond; length as m-dash" src="http://cdn.els-cdn.com/sd/entities/dbnd" class="glyphImg">pt>O) within collagen fibers of PADM. The investigations of surface interactions between PDA and PADM illustrated that PDA-PADM system yielded better mechanical
pro
perties, thermal stability, surface hydro
philicity and the structural integrity of PADM was maintained after do
pamine coating. Furthermore, collagen (COL) was immobilized onto the fresh PDA-PADM to fabricate the collagen-PDA-PADM (COL-PDA-PADM) com
plexed scaffold. The MTT assay and CLSM observation showed that COL-PDA-PADM had better biocom
patibility and higher cellular attachment than
pure PADM and COL-PADM without do
pamine coating, thus demonstrating the efficacy of PDA as the intermediate layer. Meanwhile, the ex
pression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) of COL-PDA-PADM were investigated by an
in vivo study. The results revealed that COL-PDA-PADM could effectively
promote bFGF and VEGF ex
pression,
possibly leading to enhancing the dura re
pairing
process. Overall, this work contributed a new insight into the develo
pment of a semi-
natural tissue engineering scaffold with high biocom
patibility and good mechanical
pro
perties.
p>
Statement of Significance
<
p id="s
p0015">Obtaining scaffolds with high biocom
patibility and good mechanical
pro
perties is still one of the most challenging issues in tissue engineering. To have excellent
in vitro and
in vivo performance, scaffolds are desired to have similar mechanical and biological
pro
perties as the
natural extracellular matrix, such as collagen based matrix. Utilizing the surface self-crosslinking and coating strategy, we successfully obtained a novel semi-
natural platform with excellent biological and mechanical
pro
perties from
porcine acellular
dermal matrix (PADM),
polydo
pamine and collagen. The results confirmed that this scaffold
platform has very excellent cellular
performance and very little toxicity/side effects
in vivo. Therefore, this semi-
natural scaffold may be an a
ppro
priate
platform for tissue engineering and this strategy would further hel
p to develo
p more robust scaffolds.