Genetic evidence for the requirement of the endocytic pathway in the uptake of coenzyme Q₆ in Saccharomyces cerevisiae

详细信息    查看全文

• 作者：Sergio Padilla-Ló ; pez ; Marí ; a Jimé ; nez-Hidalgo ; Alej ; ro Martí ; n-Montalvo ; Catherine F. Clarke ; Plá ; cido Navas ; Carlos Santos-Ocañ ; a
• 关键词：Coenzyme Q ; Ubiquinone ; Endomembrane ; Endocytosis ; Q uptake ; Mitochondrial disease
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：1788
• 期：6
• 页码：1238-1248
• 全文大小：1166 K

文摘

Coenzyme Q is an isoprenylated benzoquinone lipid that functions in respiratory electron transport and as a lipid antioxidant. Dietary supplementation with Q is increasingly used as a therapeutic for treatment of mitochondrial and neurodegenerative diseases, yet little is known regarding the mechanism of its uptake. As opposed to other yeast backgrounds, EG103 strains are unable to import exogenous Q₆ to the mitochondria. Furthermore, the distribution of exogenous Q₆ among endomembranes suggests an impairment of the membrane traffic at the level of the endocytic pathway. This fact was confirmed after the detection of defects in the incorporation of FM4-64 marker and CPY delivery to the vacuole. A similar effect was demonstrated in double mutant strains in Q₆ synthesis and several steps of endocytic process; those cells are unable to uptake exogenous Q₆ to the mitochondria and restore the growth on non-fermentable carbon sources. Additional data about the positive effect of peptone presence for exogenous Q₆ uptake support the hypothesis that Q₆ is transported to mitochondria through an endocytic-based system.