Rapid detection of Bombyx mori nucleopolyhedrovirus (BmNPV) by loop-mediated isothermal amplification assay combined with a lateral flow dipstick method
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- 作者：Yang Zhoua ; 1 ; Jiege Wub ; 1 ; Feng Linc ; Naifu Chend ; Shaofei Yuana ; Lina Dinga ; Li Gaoa ; Bangxing Hangd ; hanbx1978@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bombyx mori nucleopolyhedrovirus (BmNPV) ; Loop-mediated isothermal amplification (LAMP) ; Lateral flow dipstick (LFD) ; Rapid detection
- 刊名：Molecular and Cellular Probes
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：29
- 期：6
- 页码：389-395
- 全文大小：1087 K
文摘
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The LAMP-LFD for detecting BmNPV was developed by amplification at 61 °C for 45 min.
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Sensitivity of LAMP-LFD was 100 times higher in comparison to PCR.
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The LAMP-LFD was specific for BmNPV.
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We applied the LAMP-LFD to detect BmNPV in the hemolymph and feces.
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The LAMP-LFD is a simple, rapid and reliable method for detection of BmNPV.