High-level biosynthesis of norleucine in E. coli for the economic labeling of proteins
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- 作者：Niklaus Anderhubera ; 1 ; Patrik Fladischera ; b ; 1 ; Mandana Gruber-Khadjawia ; c ; 2 ; Juergen Mairhoferd ; e ; Gerald Striednera ; d ; Birgit Wiltschia ; birgit.wiltschi@acib.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：ALS ; acetolactate synthase ; cAA ; canonical amino acid ; FRT ; Flp recombinase target ; 2KB ; 2-ketobutyrate ; KO ; knockout ; M9 MM ; M9 minimal medium ; MetRS ; methionyl-tRNA synthetase ; ncAA ; non-canonical amino acid ; Nle ; norleucine ; Nva ; norvaline ; OYE ; old yellow enzyme ; RMD ; relative mean deviation ; SPI ; supplementation incorporation ; TTL ; thermophilic lipase of Thermoanaerobacter thermohydrosulfuricus ; YE ; yeast extract
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 October 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：100-111
- 全文大小：2005 K
文摘
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Metabolic engineering of E. coli to biosynthesize the methionine analog norleucine for the cost-effective labeling of proteins at large scales.
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The scalable biosynthesis of norleucine from shake flask to bioreactor cultures.
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Bioprocess engineering boosted the norleucine titers to 5 g/L.