Septin 7, nonmuscle myosin heavy chain IIA (NMHC-IIA) and SNAP23 form a complex. Knockdown of septin 7 increases NM-IIA activity in the SNAP23 complex. Insulin decreases septin 7 level and increases NM-IIA activity in the SNAP23 complex. Septin 7 hinders GSV docking/fusion by reducing NM-IIA activity in the SNAP23 complex.