Designer SiO₂@Au nanoshells towards sensitive and selective detection of small molecules in laser desorption ionization mass spectrometry

详细信息    查看全文

• 作者：Jinrui Gan ; PhD^a ; Xiang Wei ; MS^b ; Yixin Li ; MS^a ; Jiao Wu ; MS^b ; Kun Qian ; PhD^b ; ^{k.qian@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Baohong Liu ; PhD^a ; ^{bhliu@fudan.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Silica-gold nanoshells ; Surface chemistry ; Laser desorption/ionization ; Mass spectrometry ; Small molecules
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：11
• 期：7
• 页码：1715-1723
• 全文大小：1374 K

文摘

We report a novel platform using optimized SiO₂@Au core&ndash;shell structures as matrices for highly efficient laser desorption/ionization mass spectrometry analysis of small biomolecules (MW < 700 Da). Owing to the designer structure, SiO₂@Au nanoshells can achieve low detection-of-limits (~ pmol&ndash;fmol) in mass spectrometry and selective laser desorption/ionization in bio-mixtures towards diverse small molecules. By further surface modification with aptamers, Apt-SiO₂@Au nanoshells allowed simultaneously targeted enrichment and detection of kanamycin with a detection limit at 200 pM. Our work not only starts new applications of SiO₂@Au nanoshells in mass spectrometry, but also contributes to advanced analysis of either a group of small molecules or one target small molecule from complex bio-samples in a pre-designed manner for bio-diagnostics.

From the Clinical Editor

Existing methods for the detection of small molecules are often not sensitive enough. Here, the authors developed aptamer functionalized SiO2@Au nanoshells for use in mass spectrometry, with very low detection limits. The new platform appeared to be simple and efficient and should be applicable in detection of clinical samples.